Ciprofloxacin determination by solid state FT-Raman spectroscopy,

  • Raman bands observed at 1708,1624, 1548, 1493, 1273, 1253, 1238, 1024, 805, 787, 752, 665, and 638 cm-1 were used for univariate calibration.
  • Correlation coefficients of the calibration curves were in the range of  0.99-0.996 and 0.991-0.9993 for band intensity and band area measurements, respectively. 
  • Detection limits were in the range of 5.5-11 and 3.2-13 % w/w for band intensity and  band area measurements, respectively. Precision ranged between 0-11 and 0.4-12 % RSD (n=3) for band intensity and band area measurements, respectively.
  • Results obtained by the proposed method, are equivalent to those acquired  through the HPLC reference method, while it does not require dissolution of the pharmaceutical dosage forms.
  • Stepwise multiple linear regression (MLR) was also applied for calibration. The model C = (0.2 ± 1.4) - (33 ± 9) x BI1545 + (57 ± 11) x BI1272, (r = 0.997) gave the best fit. Relative differences between the FT-Raman and the reference method were in the range of  (-5.7) - (10.7) %.
  • The proposed FT-Raman method has the advantage of being fast, simple and can be used to replace tedious and time-consuming methods.

Raman spectra acquired during calibration

Standards of
(a) 0, (b) 10, (c) 30, (d) 50, (e) 70, (f) 90 and (g) 100 % w/w

Questions and comments to author: Dr C.A. Georgiou, cag@aua.gr
Phone: +301-5294248, fax: +301-5294265
Chemistry Laboratory, Agricultural University of Athens, Greece