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Raman bands observed at 554, 604, 631, 1562 and 2971 cm-1 are used for calibration. Calibration curves are linear with correlation coefficients of 0.992-0.9992 and 0.99-0.999 for band intensity and band area measurements, respectively. The linear range is 0.2-3.5 M for the 554, and 2971 cm-1 bands, 0.3-3.5 M for the 604 cm-1 band, 0.6-3.5 M for the 1562 cm-1 band and 1.0-3.5 M for the 631 cm-1 band. Detection limits are 0.1-0.3 M and 0.1-0.4 M for band intensity and band area measurements, respectively. Precision values range between 0.2-7.8 and 0.1-7.2 % RSD (n=4) for band intensity and band area measurements, respectively. The long-term calibration stability is enhanced by normalization against the 802 cm-1 cyclohexane band, compensating excitation intensity fluctuations and sample positioning changes. Results obtained by the proposed FT-Raman method, are equivalent to those acquired through the reference HPLC method. The proposed FT-Raman method is fast, simple, safe, avoids sample pre-treatment and minimises handling of toxic samples.
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