Diazinon Determination by FT-Raman Spectroscopy

  • Raman bands observed at 554, 604, 631, 1562 and 2971 cm-1 are used for calibration.
  • Calibration curves are linear with correlation coefficients of 0.992-0.9992 and 0.99-0.999 for band intensity and band area measurements, respectively. The linear range is 0.2-3.5 M for the 554, and 2971 cm-1 bands,  0.3-3.5 M for the 604 cm-1 band, 0.6-3.5 M for the 1562 cm-1 band and 1.0-3.5 M for the 631 cm-1 band.
  • Detection limits are 0.1-0.3 M and 0.1-0.4 M for band intensity and band area measurements, respectively. Precision values range between 0.2-7.8 and 0.1-7.2 % RSD (n=4) for band intensity and band area measurements, respectively.
  • The long-term calibration stability is enhanced by normalization against the 802 cm-1 cyclohexane band, compensating excitation intensity fluctuations and sample positioning changes.
  • Results obtained by the proposed FT-Raman method, are equivalent to those acquired through the reference HPLC method.
  • The proposed FT-Raman method is fast, simple, safe, avoids sample pre-treatment and minimises handling of toxic samples.

Raman spectrum of  diazinon

As recorded at 1.01, 0.81 and 0.57 W laser excitation power before (A) and after (B) normalization.

Questions and comments to author: Dr C.A. Georgiou, cag@aua.gr
Phone: +3010-5294248, fax: +3010-5294265
Chemistry Laboratory, Agricultural University of Athens, Greece