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ANALYTICAL LETTERS, 1992, VOL. 25, PP. 2305-2327
Automation of the Batch Method for Reaction Kinetic Studies Using Flow Injection Analysis. Kinetic Study of Hydrolysis of N4-Acetylsulfanilamide, Acetylsalicylic Acid and Phenyl Phosphate
Michael A. Koupparis*, Paraskevi I. Anagnostopoulou, Constantinos A. Georgiou and Themistocles P. Hadjiioannou
Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Panepistimiopolis, Athens 157 71, Greece
The automation of the discontinuous (batch) method for kinetic studies using flow-injection analysis (FIA) is described. Aliquots of the reaction mixture are automatically injected in an appropriate manifold and the kinetic profile of the reaction is obtained as a series of absorbance peaks. Observed reaction rate constants are calculated using the Guggenheim and non-linear fitting approaches. The new method is evaluated in the kinetic study of the acid hydrolysis of N4-acetylsulfanilamide by spectrophotometric monitoring of sulfanilamide, the alkaline hydrolysis of acetylsalicylic acid (aspirin) by spectrophotometric monitoring of salicylate, and the enzymic hydrolysis of phenyl phosphate with alkaline phosphatase by spectrophotometric monitoring of phosphate. The automated flow-injection batch method can be used in kinetic studies of reactions with t1/2 greater than 200 s.
Keywords: Automated batch kinetic method; flow-injection analysis; N4-acetyisulfanilamide hydrolysis; acetylsalicylic acid hydrolysis; phenyl phosphate enzymic hydrolysis; alkaline phosphatase
*Corresponding author.
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