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APPLIED SPECTROSCOPY, 2000, VOL. 55, PP. 1259-1265
Rapid Quantitative Determination of Ciprofloxacin in Pharmaceuticals Using Solid-state FT-Raman Spectroscopy
Stavroula G. Skoulika, Constantinos A. Georgiou*
Chemistry Laboratory, Agricultural University of Athens, 75 Iera Odos, 11855 Athens, Greece
Full Article-PDF (249 K)
FT-Raman spectroscopy based on band intensity or band area measurements was used for the quantitative determination of ciprofloxacin in pharmaceutical solid dosage forms. Univariate calibration was used for quantitative analysis. Bands observed at 1708, 1624, 1548, 1493, 1273, 1253, 1238, 1024, 805, 787, 752, 718, 665 and 638 cm-1 were used. Calibration curves were linear in the concentration range of 3 - 100 % w/w with correlation coefficients : 0.99-0.996 and 0.991-0.9993 for band intensity and band area measurements, respectively. Precision ranged from 0 - 11 and 0.4 - 12 % relative standard deviation (RSD) (n = 3) for band intensity and band area measurements, respectively and results were in good agreement to the current United States Pharmacopoeia (USP 24) and National Formulary (NF 19) method. Multivariate calibration was also used for quantitative analysis. Multiple linear regression using the intensities of the 1545 and 1272 cm-1 bands gave results in accordance to those obtained by the current United States Pharmacopoeia (USP 24) and National Formulary (NF 19) method. The developed solid-state FT-Raman method does not require dissolution of solid dosage forms. The proposed method can be used to replace tedious and time-consuming methods, as measurement takes just 30 s using the analytical readout from a single band.
Keywords: Ciprofloxacin; pharmaceuticals; solids; FT-Raman; quantitative; determination.
* Corresponding author.
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