Personal Profile
Nikolaos E. Labrou was born
on March 22, 1970. He received the Chemistry degree from University of Patras
(Greece) in 1991 and the doctoral degree from the Agricultural University of
Athens (Greece) in 1997. He has conducted post-doctoral research in Leeds
University (U.K., 1996-1998) and he is currently Assistant Professor in
Agricultural University of Athens. The central theme of his research has been
the development of affinity technology for enzyme purification, and enzyme
engineering to create enzymes with improved properties. His work covers
aspects of the entire range from pure to applied enzymology. He has addressed
questions regarding affinity chromatography and the structure/function of a
number of enzyme systems, using a combination of techniques on protein
engineering, protein chemistry, structural modelling and molecular dynamics
simulations. He has over 40 publications and he is actively involved in many
international collaborations.
Contact details:
Phone: +30. 210.5294308, fax: +30.210.5294308, e-mail:
lambrou@aua.gr
Address:
Laboratory of Enzyme Technology
Department of Agricultural Biotechnology,
Agricultural University of Athens,
75 Iera Odos Street, GR-118 55 Athens, Greece.
Research
Interests:
·
Enzyme engineering and modification
· Downstream processing of
enzymes
· Affinity
ligand design
Other
Activities:
Member of the editorial board of the scientific
journal
Biomolecular Engineering (Elsevier, NL).
Member of the European Federation of Biotechnology
Member of the Biochemical Society
Member of the Hellenic Society of Biochemistry
& Molecular Biology
Member of the Hellenic Chemical Society
PUBLICATIONS
A.
Research papers:
1. N.E. Labrou
and Y.D. Clonis (1995). The interaction of Candida boidinii
formate dehydrogenase with a new family of chimeric biomimetic dye-ligands. Arch.
Biochem. Biophys. 316, 169-178.
2. N.E. Labrou
and Y.D. Clonis (1995). Biomimetic affinity ligands for
oxalate-recognizing enzymes. Studies with oxalate oxidase and oxalate
decarboxylase. J. Biotechnol., 40, 59-70.
3. N.E. Labrou
and Y.D. Clonis (1995). Oxaloacetate decarboxylase: on the mode of
interaction with substrate-mimetic affinity ligands. Arch. Biochem.
Biophys. 321, 61-70.
4. N.E. Labrou and Y.D.
Clonis (1995). Biomimetic-dye affinity chromatography
for the purification of
bovine heart L-lactate dehydrogenase. J. Chromatogr., 718, 35-44.
5. N.E. Labrou,
A. Karagouni and Y.D. Clonis (1995). Biomimetic-dye affinity adsorbents
for enzyme purification: application to the one-step purification of of Candida
boidinii formate dehydrogenase. Biotech. Bioeng., 48, 278-288.
6. N.E. Labrou
E. Eliopoulos and Y.D. Clonis (1996). Dye-affinity labelling of bovine
heart mitochondrial malate dehydrogenase and study of the NADH-binding site. Biochem.
J., 315, 687-693.
7. N.E. Labrou
E. Eliopoulos and Y.D. Clonis (1996). Molecular modeling for the design
of dye-ligands and their interaction with mitochondrial malate dehydrogenase.
Biochem. J., 315, 695-703.
8. N.E. Labrou and Y.D. Clonis (1996). Biomimetic-dye
affinity chromatography for the purification of mitochondrial L-malate
dehydrogenase from bovine heart. J. Biotechnol., 45, 185-194.
9. N.E. Labrou and Y.D. Clonis (1997).
Biomimetic-dye affinity chromatography for simultaneous separation and
purification of lactate dehydrogenase and malate dehydrogenase from bovine
heart. Bioprocess Engineering, 16, 157-161.
10. N.E. Labrou and Y.D. Clonis (1997). L-malate
dehydrogenase from Pseudomonas stutzeri: purification and
characterization.
Arch. Biochem. Biophys., 337, 103-114.
11.
N.E. Labrou (1998). Candida boidinii, fdh2 gene. EMBL,
European Bioinformatics Institute, Nucleotide Sequence Database, Accession
No: AJ011046.
12. N.E. Labrou,
E. Eliopoulos and Y.D. Clonis (1999). Molecular Modelling for the Design
of a Biomimetic Chimeric Ligand. Application to the Purification of Bovine
Heart L-Lactate dehydrogenase. Biotechnol. Bioeng. 63, 322-332.
13.
N.E. Labrou, L.V. Mello, D.J. Rigden, J.N. Keen & J.B.C. Findlay (1999).
Structure-activity studies on cysteine-substituted neurokinin A analogs. Peptides,
20, (7), 795-801.
14. N.E. Labrou and Y.D. Clonis (1999).
Oxaloacetate decarboxylase from Pseudomonas stutzeri: purification and
characterization. Arch. Biochem. Biophys., 365, 17-24.
15.
N.E. Labrou (1999). Afinity labeling of oxaloacetate decarboxylase by
novel dichlorotriazine linked ?-ketoacids.
J. Prot. Chem., 18, 729-733.
16.
N.E. Labrou (2000). Improved Purification of Candida boidinii
Formate Dehydrogenase. Bioseparation, 9, 99-104.
17.
N.E. Labrou (2000). Dye Affinity Labelling of yeast Alcohol
Dehydrogenase. Enzyme Inhibition, 15, 487-496.
18. N.
E. Labrou, D. J. Rigden & Y. D. Clonis (2000) Characterization of NAD+
binding site of Candida boidinii Formate Dehydrogenase by affinity
labelling and site-directed mutagenesis. Eur. J. Biochem. 267,
6657-6664..
19. N.
E. Labrou & D. J. Rigden (2001) Active site characterization of Candida
boidinii Formate Dehydrogenase. Biochem. J. 354, 455-463.
20. N.E.
Labrou, L.V. Mello and Y.D. Clonis (2001). Structural and
functional role of the glutathione binding residues in maize glutathione
S-transferase. Biochem. J., 358,
101-10.
21. N.E.
Labrou, L.V. Mello and Y.D. Clonis (2001). The conserved Asn49 in maize
glutathione S-transferase modulates substrate binding, catalysis and
intrasubunit communication. Eur. J. Biochem., 268, 3950-7.
22. N.E. Labrou, Bhogal, N.,
Hurrell, CR, J.B. Findlay (2001). Interaction of Met297 in the seventh
transmembrane segment of the tachykinin NK2 receptor with neurokinin A. J Biol Chem. 276, 37944-9.
B.
Reviews
1. N.E. Labrou and Y.D. Clonis (1994). The
affinity technology in downstream processing. J. Biotechnol., 36,
95-119.
2.
Y.D. Clonis, N.E. Labrou, V. Kotsira, K. Mazitsos, S. Melissis, & G.
Gogolas. (2000). Biomimetic Dyes as Affinity Chromatography Tools in
Enzyme Purification. J. Chromatogr. 891, 33-44.
3. N.E. Labrou (2002). Dye-ligand affinity adsorbents for enzyme purification. Mol.
Biotechnology, 20, 77-84.
C. Chapters in Books
1.
N.E. Labrou and Y.D. Clonis (1999). The Tachykinin Family of Peptides
and Their Receptors. In Matsoukas J. & Mavromoustakos (Eds), ‘Bioctive
Peptides in Drug Discovery and Design: Medical Aspects’. IOS
Press, Amsterdam, The Netherlands. 225-233.
2.
N.E. Labrou (2000). Dye-ligand Affinity Chromatography for Protein
Separation and Purification. In: Walker, J.M. (Ed), Methods in Molecular
Biology: ‘Affinity Chromatography: Methods and Protocols’. Humana
Press, USA, vol. 147, 129-139.
3. N. Bhogal, N.E. Labrou, R. N. Moser, D. Donnelly, C.R. Hurrel, A.
Cox, and J. B. C. Findlay (2001). Mapping of the Ligand
Binding Domain of the Human Tachykinin NK2 Receptor. In:
Girdlestone, D. (Ed.), Receptor Μechanisms Series. IUPHAR Μedia Publishers,
London, UK. (in press).
4. N.E. Labrou & J.B.C.
Findlay (2000). Interaction of
cysteine-substituted Neurokinin A analogues with human Neurokinin-2 receptor.
2nd Forum on Bioactive Peptides. Ed.:
P. Kordopatis, Typorama, Patra, Greece.
5.
N.E. Labrou and Y.D. Clonis (2002). Immobilised Synthetic Dyes in
Affinity Chromatography. Biochromatography
- Theory and Practice
(M.A. Vijayalakshmi, ed.), Taylor and Francis Publishers, London, Chap.
8, pp 235-251, 2002.
6. N.E. Labrou. Affinity Chromatography. In Gupta,
M. N. (Ed.), Methods for Affinity-Based Separations Of Enzymes and Proteins.
Birkhϊuser
Verlag AG, Switzerland, (in press).
7. N.E. Labrou, K. Mazitsos and Y. Clonis. Dye-ligand and Biomimetic
Affinity Chromatography. In D.S. Hage (Ed.), Handbook of Affinity
Chromatography. Marcel Dekker, Inc, New York, (in press).
8. N.E. Labrou, Axarli, I.,
Melissis, S., Kotzia, G., Katsos, N. & Y.D. Clonis. Glutathione S-transferase: de novo design of glutathionyl-analogues and combinatorial design
of novel enzyme reactions. 3rd Forum on
Bioactive Peptides. Ed.: P. Kordopatis, Typorama, Patra, (in press).
D. Proceedings of Scientific Conferences
1.
Makrigiannis T., Dionisopoulou, E, N. Labrou, Manesi-Zoupa, E.,
Kordopatis, P., & Clonis, Y. (1992). Immobilized Biomimetic Dyes and
Application in Enzyme Purification. Proceedings
of the 6th Hellenic
Pharmaceutical Symposium, June, Athens, Greece.
2. N.E.
Labrou and Y.D. Clonis (1995) Design, synthesis and characterization of
new chimeric biomimetic dye-ligands for carboxyl-group-recognizing enzymes.
Application to the one step-purification of L-malate dehydrogenase with
affinity chromatography. Proceedings of
the 42nd Hellenic Biochemical and
Biophysical Society, 13-14 January, Athens, Greece.
3. N.E.
Labrou and Y.D. Clonis (1996). Affinity labelling of nucleotide-dependent
enzymes and proteases with dichlorotriazine dyes. Proceedings of the 43nd
Hellenic Biochemical and Biophysical Society, 13-14 January, Athens,
Greece.
4. N. Labrou, E. Eliopoulos, Y. Clonis (1998). Molecular Modelling for the Study of Protein-Ligand Interaction
and Ligand Design. Application to the Case of a Biomimetic Bifunctional
Ligand for the Purification of Bovine Heart Lactate Dedydrogenase. Proceedings of the 2nd European Symposium on Biochemical
Engineering Science. Porto, Portugal, 16-19 September.
5. N. Labrou,
V. Kotsira, K. Mazitsos, G. Gogolas and Y. Clonis (1999). Biomimetic
dye-ligands in enzyme purification. Proceedings
of the 13th International Symposium
on Affinity Technology and Bio-Recognition, July 4-8, Compiegne, France.
6. N.E. Labrou & J.B.C.
Findlay (2000). Interaction of
cysteine-substituted Neurokinin A analogues with human Neurokinin-2 receptor.
Proceedings of the 2nd Hellenic Forum
on Bioactive Peptides. Conference and Cultural center of the University of
Patras. April 14-15, Patra, (SL-02).
7. N. E.
Λάμπρου, Γ. Κοτζιά, Π. Νάσης, & Ι. Κλώνης (2001). Ανασχεδιασμός της
εκλεκτικότητας του ενζύμου μεταφοράση του S-γλουταθειου
από αραβόσιτο και τροποποίηση της εκλεκτικότητας του για το ξενοβιοτικό
υπόστρωμα. Πρακτικά του 23ου Συνεδρίου της Ελληνικής Εταιρίας Βιολογικών
Επιστημών, 24-27 Μαΐου, Χίος, (υπό εκτύπωση).
8. N.E.
Labrou and Y.D. Clonis (2001) Protein engineering of Candida boidinii formate dehydrogenase: cloning, expression and
redesign of coenzyme specificity. Proceedings
of the 53rd Hellenic Biochemical
and Biophysical Society, 13-15 December, Athens, Greece.
9. N.E.
Labrou & Y.D. Clonis (2002) Glutathione S-transferase: de novo
design of glutathionyl-analogues and combinatorial design of novel enzyme
reactions. 3rd Hellenic Forum on
Bioactive Peptides. Conference and Cultural center of the University of
Patras. April 11-13, Patra, Greece (SL 08).
10. Clonis, YD., Labrou,
N.E., Andreou, V., Melissis, S., Kotzia, G., Nasis, P., Dourou, T.,
Voutsinos, A. (2002) An enzymatic technological triangle: Biomimetic
molecules, enzyme engineering and biosensors. 3rd Hellenic Forum on Bioactive Peptides. Conference and Cultural center
of the University of Patras. April 11-13, Patra, Greece (P-55).
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