LABORATORY OF ENZYME TECHNOLOGY

 

 

 

 

 

 

 

 

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Personal Profile

 

Nikolaos E. Labrou was born on March 22, 1970. He received the Chemistry degree from University of Patras (Greece) in 1991 and the doctoral degree from the Agricultural University of Athens (Greece) in 1997. He has conducted post-doctoral research in Leeds University (U.K., 1996-1998) and he is currently Assistant Professor in Agricultural University of Athens. The central theme of his research has been the development of affinity technology for enzyme purification, and enzyme engineering to create enzymes with improved properties. His work covers aspects of the entire range from pure to applied enzymology. He has addressed questions regarding affinity chromatography and the structure/function of a number of enzyme systems, using a combination of techniques on protein engineering, protein chemistry, structural modelling and molecular dynamics simulations. He has over 40 publications and he is actively involved in many international collaborations.

 

Contact details:

Phone: +30. 210.5294308, fax: +30.210.5294308, e-mail: lambrou@aua.gr

 

Address:

Laboratory of Enzyme Technology

Department of Agricultural Biotechnology,

Agricultural University of Athens, 

75 Iera Odos Street, GR-118 55 Athens, Greece.

 

Research Interests: 

·       Enzyme engineering and modification

·       Downstream processing of enzymes

 

·       Affinity ligand design

 

Other Activities:

Member of the editorial board of the scientific journal

Biomolecular Engineering (Elsevier, NL).

Member of the European Federation of Biotechnology

Member of the Biochemical Society

Member of the Hellenic Society of Biochemistry & Molecular Biology

Member of the Hellenic Chemical Society

PUBLICATIONS

A. Research papers:

1.  N.E. Labrou and Y.D. Clonis (1995). The interaction of Candida boidinii formate dehydrogenase with a new family of chimeric biomimetic dye-ligands. Arch. Biochem. Biophys. 316, 169-178.

 

2.  N.E. Labrou and Y.D. Clonis (1995). Biomimetic affinity ligands for oxalate-recognizing enzymes. Studies with oxalate oxidase and oxalate decarboxylase. J. Biotechnol., 40, 59-70.

 

3.  N.E. Labrou and Y.D. Clonis (1995). Oxaloacetate decarboxylase: on the mode of interaction with substrate-mimetic affinity ligands. Arch. Biochem. Biophys. 321, 61-70.

 

4. N.E. Labrou and Y.D. Clonis (1995). Biomimetic-dye affinity chromatography 

for the purification of bovine heart L-lactate dehydrogenase. J. Chromatogr., 718, 35-44.

 

5.  N.E. Labrou, A. Karagouni and Y.D. Clonis (1995). Biomimetic-dye affinity adsorbents for enzyme purification: application to the one-step purification of of Candida boidinii formate dehydrogenase. Biotech. Bioeng., 48, 278-288.

 

6.  N.E. Labrou E. Eliopoulos and Y.D. Clonis (1996). Dye-affinity labelling of bovine heart mitochondrial malate dehydrogenase and study of the NADH-binding site. Biochem. J., 315, 687-693.

 

7.  N.E. Labrou E. Eliopoulos and Y.D. Clonis (1996). Molecular modeling for the design of dye-ligands and their interaction with mitochondrial malate dehydrogenase. Biochem. J., 315, 695-703.

 

8. N.E. Labrou and Y.D. Clonis (1996). Biomimetic-dye affinity chromatography for the purification of mitochondrial L-malate dehydrogenase from bovine heart. J. Biotechnol., 45, 185-194.

 

9. N.E. Labrou and Y.D. Clonis (1997). Biomimetic-dye affinity chromatography for simultaneous separation and purification of lactate dehydrogenase and malate dehydrogenase from bovine heart. Bioprocess Engineering, 16, 157-161.
 

10. N.E. Labrou and Y.D. Clonis (1997). L-malate dehydrogenase from Pseudomonas stutzeri: purification and

characterization. Arch. Biochem. Biophys., 337, 103-114.

11. N.E. Labrou (1998). Candida boidinii, fdh2 gene. EMBL, European Bioinformatics Institute, Nucleotide Sequence Database, Accession No: AJ011046.

 

12. N.E. Labrou, E. Eliopoulos and Y.D. Clonis (1999). Molecular Modelling for the Design of a Biomimetic Chimeric Ligand. Application to the Purification of Bovine Heart L-Lactate dehydrogenase. Biotechnol. Bioeng. 63, 322-332.

 

13. N.E. Labrou, L.V. Mello, D.J. Rigden, J.N. Keen & J.B.C. Findlay (1999). Structure-activity studies on cysteine-substituted neurokinin A analogs. Peptides, 20, (7), 795-801.

 

14. N.E. Labrou and Y.D. Clonis (1999). Oxaloacetate decarboxylase from Pseudomonas stutzeri: purification and characterization. Arch. Biochem. Biophys., 365, 17-24.

 

15. N.E. Labrou (1999). Afinity labeling of oxaloacetate decarboxylase by novel dichlorotriazine linked ?-ketoacids. J. Prot. Chem., 18, 729-733.

 

16. N.E. Labrou (2000). Improved Purification of Candida boidinii Formate Dehydrogenase. Bioseparation, 9, 99-104.

 

17. N.E. Labrou (2000). Dye Affinity Labelling of yeast Alcohol Dehydrogenase. Enzyme Inhibition, 15, 487-496.

 

18. N. E. Labrou, D. J. Rigden & Y. D. Clonis (2000) Characterization of NAD+ binding site of Candida boidinii Formate Dehydrogenase by affinity labelling and site-directed mutagenesis. Eur. J. Biochem. 267, 6657-6664..

 

19. N. E. Labrou & D. J. Rigden (2001) Active site characterization of Candida boidinii Formate Dehydrogenase. Biochem. J. 354, 455-463.

20. N.E. Labrou, L.V. Mello and Y.D. Clonis (2001). Structural and functional role of the glutathione binding residues in maize glutathione S-transferase. Biochem. J., 358, 101-10.

 

21. N.E. Labrou, L.V. Mello and Y.D. Clonis (2001). The conserved Asn49 in maize glutathione S-transferase modulates substrate binding, catalysis and intrasubunit communication. Eur. J. Biochem., 268, 3950-7.

 

22. N.E. Labrou, Bhogal, N., Hurrell, CR, J.B. Findlay (2001). Interaction of Met297 in the seventh transmembrane segment of the tachykinin NK2 receptor with neurokinin A. J Biol Chem. 276, 37944-9.

 

 

B. Reviews

 

1. N.E. Labrou and Y.D. Clonis (1994). The affinity technology in downstream processing. J. Biotechnol., 36, 95-119.

 

2. Y.D. Clonis, N.E. Labrou, V. Kotsira, K. Mazitsos, S. Melissis, & G. Gogolas. (2000). Biomimetic Dyes as Affinity Chromatography Tools in Enzyme Purification. J. Chromatogr. 891, 33-44.

 

3. N.E. Labrou (2002). Dye-ligand affinity adsorbents for enzyme purification. Mol. Biotechnology, 20, 77-84.

 

C. Chapters in Books

 

1. N.E. Labrou and Y.D. Clonis (1999). The Tachykinin Family of Peptides and Their Receptors. In Matsoukas J. & Mavromoustakos (Eds), ‘Bioctive Peptides in Drug Discovery and Design: Medical Aspects’. IOS Press, Amsterdam, The Netherlands. 225-233.

 

2. N.E. Labrou (2000). Dye-ligand Affinity Chromatography for Protein Separation and Purification. In: Walker, J.M. (Ed), Methods in Molecular Biology:Affinity Chromatography: Methods and Protocols’. Humana Press, USA, vol. 147, 129-139.

 

3. N. Bhogal, N.E. Labrou, R. N. Moser, D. Donnelly, C.R. Hurrel, A. Cox, and J. B. C. Findlay (2001)Mapping of the Ligand Binding Domain of the Human Tachykinin NK2 Receptor. In: Girdlestone, D. (Ed.), Receptor Μechanisms Series. IUPHAR Μedia Publishers, London, UK. (in press).

4. N.E. Labrou & J.B.C. Findlay (2000). Interaction of cysteine-substituted Neurokinin A analogues with human Neurokinin-2 receptor. 2nd Forum on Bioactive Peptides. Ed.: P. Kordopatis, Typorama, Patra, Greece.

 

5. N.E. Labrou and Y.D. Clonis (2002). Immobilised Synthetic Dyes in Affinity Chromatography. Biochromatography - Theory and Practice (M.A. Vijayalakshmi, ed.), Taylor and Francis Publishers, London, Chap. 8, pp 235-251, 2002.

 

6. N.E. Labrou. Affinity Chromatography. In Gupta, M. N. (Ed.), Methods for Affinity-Based Separations Of Enzymes and Proteins. Birkhϊuser Verlag AG, Switzerland, (in press).

 

7.  N.E. Labrou, K. Mazitsos and Y. Clonis. Dye-ligand and Biomimetic Affinity Chromatography. In D.S. Hage (Ed.), Handbook of Affinity Chromatography. Marcel Dekker, Inc, New York, (in press).

 

8. N.E. Labrou, Axarli, I., Melissis, S., Kotzia, G., Katsos, N. & Y.D. Clonis. Glutathione S-transferase: de novo design of glutathionyl-analogues and combinatorial design of novel enzyme reactions. 3rd Forum on Bioactive Peptides. Ed.: P. Kordopatis, Typorama, Patra, (in press).

 

D. Proceedings of Scientific Conferences

1. Makrigiannis T., Dionisopoulou, E, N. Labrou, Manesi-Zoupa, E., Kordopatis, P., & Clonis, Y. (1992). Immobilized Biomimetic Dyes and Application in Enzyme Purification. Proceedings of the 6th Hellenic Pharmaceutical Symposium, June, Athens, Greece. 

 

2. N.E. Labrou and Y.D. Clonis (1995) Design, synthesis and characterization of new chimeric biomimetic dye-ligands for carboxyl-group-recognizing enzymes. Application to the one step-purification of L-malate dehydrogenase with affinity chromatography. Proceedings of the 42nd Hellenic Biochemical and Biophysical Society, 13-14 January, Athens, Greece.

 

3. N.E. Labrou and Y.D. Clonis (1996). Affinity labelling of nucleotide-dependent enzymes and proteases with dichlorotriazine dyes. Proceedings of the 43nd Hellenic Biochemical and Biophysical Society, 13-14 January, Athens, Greece.

 

4. N. Labrou, E. Eliopoulos, Y. Clonis (1998). Molecular Modelling for the Study of Protein-Ligand Interaction and Ligand Design. Application to the Case of a Biomimetic Bifunctional Ligand for the Purification of Bovine Heart Lactate Dedydrogenase. Proceedings of the 2nd European Symposium on Biochemical Engineering Science. Porto, Portugal, 16-19 September.

 

5. N. Labrou, V. Kotsira, K. Mazitsos, G. Gogolas and Y. Clonis (1999). Biomimetic dye-ligands in enzyme purification. Proceedings of the 13th International Symposium on Affinity Technology and Bio-Recognition, July 4-8, Compiegne, France.

 

6. N.E. Labrou & J.B.C. Findlay (2000). Interaction of cysteine-substituted Neurokinin A analogues with human Neurokinin-2 receptor. Proceedings of the 2nd Hellenic Forum on Bioactive Peptides. Conference and Cultural center of the University of Patras. April 14-15, Patra, (SL-02).

 

7. N. E. Λάμπρου, Γ. Κοτζιά, Π. Νάσης, & Ι. Κλώνης (2001). Ανασχεδιασμός της εκλεκτικότητας του ενζύμου μεταφοράση του S-γλουταθειου από αραβόσιτο και τροποποίηση της εκλεκτικότητας του για το ξενοβιοτικό υπόστρωμα. Πρακτικά του 23ου Συνεδρίου της Ελληνικής Εταιρίας Βιολογικών Επιστημών, 24-27 Μαΐου, Χίος, (υπό εκτύπωση).

 

8. N.E. Labrou and Y.D. Clonis (2001) Protein engineering of Candida boidinii formate dehydrogenase: cloning, expression and redesign of coenzyme specificity. Proceedings of the 53rd Hellenic Biochemical and Biophysical Society, 13-15 December, Athens, Greece.

 

9. N.E. Labrou & Y.D. Clonis (2002) Glutathione S-transferase: de novo design of glutathionyl-analogues and combinatorial design of novel enzyme reactions. 3rd Hellenic Forum on Bioactive Peptides. Conference and Cultural center of the University of Patras. April 11-13, Patra, Greece (SL 08).

 

10. Clonis, YD., Labrou, N.E., Andreou, V., Melissis, S., Kotzia, G., Nasis, P., Dourou, T., Voutsinos, A. (2002) An enzymatic technological triangle: Biomimetic molecules, enzyme engineering and biosensors. 3rd Hellenic Forum on Bioactive Peptides. Conference and Cultural center of the University of Patras. April 11-13, Patra, Greece (P-55).

 

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