WebCutter: A free, on-line program to help restriction map DNA sequences

What is Webcutter?
Webcutter is an on-line tool for restriction mapping nucleotide sequences. It features:
September 30: Swedish mirror
This mirror site was established to supplement the original site at http://firstmarket.com/firstmarket/cutter in the United States.
Many thanks to Tore Samuelsson at the University of Goteburg for his generous donation of processor space, and his assistance in building the mirror
August 29: Frequency ranges
The option of searching for enzymes that cut within a range of frequencies was added
Thanks to Klaus Salger at the University of Meunchen for the idea
August 18: SeqSeek
SeqSeek, a script for direct sequence retrieval from NCBI's GenBank, was written and integrated into WebCutter
Thanks to David Landsman at the National Institute of Health for the idea
If there's a feature you'd like to see in WebCutter, please suggest it!

How to use Webcutter
There are three ways to enter your sequence:

On the bottom half of this page, you will find ways to customize your digest. If you experience any problem with Webcutter or if you have any suggestions for its improvement, please let the author know.


Please enter a title for this sequence:

Paste the DNA sequence into the box below

Please indicate what kind of sequence analysis you would like:

Map of restriction sites

Table of site positions
Arrange the table alphabetically by enzyme, or sequentially by position of site?

Please indicate which enzymes to use:


Credits
Webcutter was written and designed by Max Heiman while working as a summer student in Dr. Steven Reeves' lab in Molecular NeuroOncology at Mass. General Hospital. The space for this copy of WebCutter was kindly provided by Dr. Tore Samuelsson at the University of Goteburg.

WebCutter is mirrored in the United States. Processor space there is borrowed from First Market Research, a firm specializing in high-technology studies. Over-the-shoulder support during WebCutter's development was furnished by Bib Sinha, David Lisle, Andy Hong, and Dr. Reeves.

Special thanks for debugging assistance go to Dana Macelis, programmer for New England BioLabs' REBASE, from which Webcutter's enzyme database is taken, and to Pedro Coutinho, maintainer of Pedro's Biomolecular Research Tools.

To the best of the author's knowledge Webcutter works great; however, use it at your own risk.


Webcutter 1.0, copyright 1995 Max Heiman. Please send suggestions to maxwell@minerva.cis.yale.edu or visit the author's home page.