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PMG-Research Team

The Pesticide Metabolomics Group, leaded by Dr K. A. Aliferis, is hosted by the Laboratory of Pesticide Science of the Agricultural University of Athens (AUA), Athens, Greece.  The group was established in 2016.

The PMG is aiming to provide solutions to major issues that the agrochemical, agricultural, and food sectors are currently facing by employing advanced metabolomics and bioinformatics as its main bioanalytical tools.

 

 PMG’s research axes include, but are not limited to, topics of:

·        Pest and pathogen Resistance to Pesticides

·         Nanopesticides

·        Pesticide Toxicity (toxicology and ecotoxicology)

·         Natural product Discovery

·         Plant-microbe Interactions

·         Plant Metabolomics

·         Pesticide Residues

·         Food Metabolomics

Current and past Members of the PMG:

Dr K. A. Aliferis (Group Leader)                                      

  

Editorial Board: Pesticide Biochemistry and Physiology: http://www.journals.elsevier.com/pesticide-biochemistry-and-physiology/
LinkedIn:
https://www.linkedin.com/pub/konstantinos-aliferis/24/b41/88
Research Gate:
http://www.researchgate.net/profile/Konstantinos_Aliferis/info

COST Participant: FA COST Action FA1306, The quest for tolerant varieties - Phenotyping at plant and cellular level

POSTDOCTORAL FELLOWS 

I. Kalambokis M.Sc., Ph.D. (Postdoctoral fellow) Fungal resistance-fungal metabolomics     

My  research focuses  on the  development of  combined genetic engineering-metabolomics  approaches within the context of systems biology, for the in-depth understudying of the mode(s)-of-action of fungicides and mechanisms of fungal resistance to fungicides.

Currently, the use of pesticides represents the main crop protection strategy for the quantitative and qualitative production of  agricultural  products at affordable prices. However, the  effectiveness of pesticides depends on the concentration of the active ingredient at the subcellular target site. This is the result of the balance between uptake, probable degradation and excretion of the active ingredient.One of the most effective control mechanisms of uptake and excretion, is the active transport through the action of membrane transport proteins, simply known as transporters. Although excretion transporters (e.g, ABC and MFS) have to some extent been studied, it is interesting that this is not the case for transporters that control the uptake of substances into the cell. Additionally, their effect on the metabolism and phenotype of organisms is largely unknown.

The relatively recent development of metabolomics and its combination with molecular genetic techniques in the context of systems biology, is able to give answers to the above biological questions. Results could be exploited in crop protection towards the development of improved crop protection agents and the assessment of the risk of fungal resistance to pesticides.

Part of the project is in collaboration with Prof. Diallinas's Aspergillus Genetics Lab, Department of BiologyNational and Kapodistrian University of Athens.

 

POSTGRADUATE STUDENTS

A.E. Papadopoulou M.Sc. (Ph.D. student): Fungal endophytes and plant protection

 

 

 

 E. Xaxiri M.Sc. (Ph.D. student): Effect of plant protection products on plant metabolism

 

 

 

 

M. Lykogianni M.Sc. (Ph.D. student): Nanopesticides-ecotoxicogenomics

            

Nanotechnology is among the most rapidly evolving and promising field of sciences. Various applications of nanotechnology have been developed in almost any scientific discipline including Agricultural Sciences and more specifically Crop Protection. The development of nanopesticides could provide solutions to problems and challenges that the crop protection sector is facing e.g., resistance of plant pathogens and insects to pesticides, lack of pesticides for several plant diseases, development of pesticides with improved efficacy and toxicological profiles.

My study is focusing on the assessment of the potential of nanoparticles to be used per se as pesticides or as nannocarriers in the formulation of nanopesticides. Their bioactivity against different fungi species is being evaluated and their toxicological effects on non-target organisms applying metabolomics is being investigated. Through this study I am aiming to provide an improved, in-depth understanding of the biochemichal mechanisms and mode(s)-of-action of nanomaterials through the employment of advanced metabolomics, which in turn could greatly assist the development of improved or alternative crop protection strategies and agents. This project is withiin the context of an on-going collaboration with research group from the Department of Physical Chemistry, National Centre for Scientific Research "Demokritos".

     

S. Kolainis (M.Sc.): Olive anthracnose: Improving plant protection through advanced metabolomics

 

 

K. Metaxa (B.Sc.): Cannabis (Cannabis sativa L.) R&D: Study of the effects of biostimulants on the plant's metabolism and cannabinoid biosynthesis applying metabolomics

Cannabis (Cannabis sativa L.), is a species exhibiting a great potential for applications in various sectors. Nonetheless, the strict regulation of PPPs applications dictate the need for the discovery and/or assessment of alternative PPPs. Within this context, biostimulants represent a source of high potential in Cannabis plant protection. My work focuses on the assessment of the effects of such products on the metabolism of the plant and its cannabinoid content applying metabolomics

 

A.-I. Bastas (B.Sc.): Cannabis (Cannabis sativa L.) R&D: Study of the toxicity of selected plant protection products on the on the plant's metabolism and cannabinoid biosynthesis applying metabolomics

Conventional plant protection products (PPPs) represent the backbone of plant protection. Nonetheless, information on their effects on plant metabolism, with the exception of herbicides, is scarce. Additionally, for species such as Cannabis (Cannabis sativa L.) the relevant information is non-existent. Based on the above, aim of my work is the study of the effects of selected fungicides on the metabolism of Cannabis and the biosynthesis of Cannabinoids and terpenoids via metabolomics  

 

E. Pantazi (M.Sc.): Medicinal plants as sources of bioactivity in plant protection

 

The plant protection sector is facing major issues and challenges. Therefore, it is very important to discover new, alternative sources of bioactivity and develop new plant protection agents and strategies. Within this context, the exploitation of bio-stimulants, natural products and endophytes in plant protection could provide valuable solution. In my research, I am evaluating the potential of extracts of the medicinal plant Aloe barbadensis to be used as a biostimulant in plant protection applying plant metabolomics.

 

S. Kostopoulou (M.Sc.): Ecotoxicogenomics-Lemna minor L.

 

Ecotoxicological and toxicological studies represent key parts of the research that is required for the development of pesticides. Currently, results of toxicological studies have indicated endocrine disrupting activity of some of the most widely applied pesticides.Ecotoxicological and toxicological studies represent key parts of the research that is required for the development of pesticides. Currently, results of toxicological studies have indicated endocrine disrupting activity of some of the most widely applied pesticides.My research is focused on the assessment of the model plant in ecotoxicological studies, duckweed (Lemna minor L.) as an indicator of the presence of herbicides acting as endocrine disruptors in aquatic environments and their mixtures, applying gas chromatography mass spectrometry (GC/MS) metabolomics and the discovery of metabolite-biomarkers of toxicity. Although the application of metabolomics in toxicogenomics is still in infancy, results of our research on endocrine disruptors’ toxicity is expected to provide new insights into the mechanism involved.

 

E. Fotopoulou (M.Sc.): Toxicogenomics-Neural Stem cells and pesticide toxicity

                    

Among the most challenging topics of pesticide toxicology is the understanding and assessment of pesticide toxicity through the study of their effects on the metabolism of biological systems. I am interested in developing robust bioanalytical protocols for the toxicological risk assessment of pesticides applying metabolomics, using as model biological system neural stem cells. Currently, the application of metabolomics in the study of stem cells is still in its infancy. My goal is by applying metabolomics to discover metabolites-biomarkers, which could serve in the early screening of bioactive compounds in the pesticide R&D pipeline. This project is in collaboration with research group from the Hellenic Pasteur Institute (HPI).

D. Karamanou (M.Sc.): Fungal resistance-Yeast metabolomics

                

One of the main mechanisms of resistance of phytopathogenic fungi to applied fungicides, is that of the increased secretion of the active ingredient. Through this mechanism, pathogens become resistant to a variety of active ingredients (Multidrug Resistance, MDR), without being predictable which of the applied fungicides will lose their effectiveness through such mechanism.

Our knowledge on increased excretion of the active ingredients could be significantly improved through the understanding of the interactions between the main biochemical pathways involved, the genotype and phenotype of resistant strains.

Our knowledge on increased excretion of the active ingredients could be significantly improved through the understanding of the interactions between the main biochemical pathways involved, the genotype and phenotype of resistant strains.

Acquiring an in-depth understanding of such mechanism could enable the management of resistant populations, and the reduction of cross resistance incidence in susceptible populations.

UNDERGRADUATE STUDENTS 

O. Anestidou (B.Sc.): Wine metabolomics: safety and authentication

 

 

 

 

C. Kerezoudis (B.Sc.): Assesing the toxicity of plant protection products to Cannabis (Cannabis sative L. ) applying metabolomics

 

 

 

 

N. Mparkolias (B.Sc.): Olive tree plant protection against fungal pathogens using advanced plant protection products

 

 

 

E. Pavlakou (B.Sc.): Cannabis (Cannabis sativa L.) plant protection against Rhizoctonia solani: evaluation of novel  sources of bioactivity via phenomics and metabolomics

 

 

 

 

 

A. Stergios (B.Sc.): Olive oil metabolomics and study of the bioactivity of olive mill waste (OMW)

The olive oil is one of the most important agricultural products of Greece, with a significant contribution to the GDP of the country and to the income of those involved in its production and marketing. However, quantitative and qualitative losses due to pest and pathogen infections, as well as issues in the marketing and distribution networks re4presents obstacles towards the further development of the sector.

The aim of my research is the development of an integrated GC/EI/MS-1HNMR metabolomics approach for the monitoring of the quality of olive oil (adulteration, residues of plant protection products, traceability) and the study of the potential of olive mill waste (OMW) from a two-phase system in olive tree plant protection.

E. Lamprakopoulou (B.Sc.): Isolation of cannabis phytopathogens and evaluation of the bioactivity of biological agents against them

Cannabis (industrial and medicinal) (Cannabis sativa L.) is an emerging cultivation world-wide. The legislation on medicinal cannabis in Greece was introduced in May of 2018. A large number of pests and pathogen cause severe quantitative and qualitative yield loses in field and greenhouse cultivations. Additionally, for the production of superior quality organic cannabis and cannabis products the use of chemical plant protection products is not permitted.

The aim of my research is the isolation of cannabis pathogens from field cultivations and the evaluation of the activity of biological agents against them.

 E. Alexandri (B.Sc.): Olive mill waste (OMW): Phytotoxictiy and bioactivity assesment

The management of olive mill waste (OMW) represents a serious challenge, due to their high content in organics, organic acids and polyphenols, which are both phytotoxic and degrade the quality of the environment. Due to their content in bioactive compounds, they represent rich sources of bioactivity for potential applications in plant protection.  

Within this context, I am studying the phytotoxicity of OMW water extracts using Lemna minor L. as the model plant.  Also, I am evaluating their effect on seed germination, their phytotoxicity to crop and weed species plants and their mycotoxicity to selected phytopathogenic fungi. 

 

E. Papadopoulou (B.Sc.): New sources of bioactivity in pesticide R&D

                    

The agrochemical and agricultural sectors are facing great challenges such as, the development of resistant to pesticides plant pathogens, the recent banning of many pesticides and the increasing public concern over food quality and safety. Among the key strategies to tackle such challenges is the discovery of new sources of bioactivity or development of new or alternative crop protection agents.

In my research, fungitoxicity and phytotoxicity bioassays are conducted in order to assess the potential of selected nanoparticles to be used i style="mso-bidi-font-style:normal">per se or as nanocarriers in nanopesticide formulation. Additionally, the bioactivity of biochar-a product produced through pyrolysis of plant residues-against different plant pathogenic fungi and bacteria will be evaluated, which is within the context of a continuous and close collaboration with a research team of McGill University, Montreal, Canada.

S. Kolainis (B.Sc.): Olive tree pathogenic fungi: Isolation, identification, and resistance screening

The recent years a significant decrease in olive oil production has been observed in Greece, leading to losses of more than 40,000 tons and /span> additionally significant losses in the yield of table olives. Such losses correspond to millions of euros of damages for the producers. In addition to the well-known pest, the olive fruit fly (Bactrocera oleae), a significant part of the losses was due to fungal infections of the fruits.

My research is focused on the isolation, identification and study of the pesticide resistance level of fungal strains isolated from infected olive fruits. Results of my research will provide insights into the issue and will propose measures for reducing future yield losses. In this project we collaborate with the Hellenic Ministry of Rural evelopment and Food.

PP. Konstantopoulou (B.Sc.): Natural product discovery

The  incorrect use  of agrochemicals  has led to issues such as, the  development of resistant to pesticides  pest and pathogens, which in  turn dictate the need for the discovery of new sources of bioactivity. The latter should have minimum impact on humans and the environment, and at the same time should offer improved crop protection.o:p>

Within this context, aim of my research is the assessment of the bioactivity of natural extracts against plant pathogenic fungi and the study of the effect of pesticides on the metabolism of selected fungi applying metabolomics.

 L. Kotsos (B.Sc.): Effects of fungicides on plant metabolism

Pesticides are applied in order to protect crops from pest and pathogens. However, there are increasing indications that crop protection products affect additionally the physiology and nutritional value of plants. This is mainly due to the fact that in addition to their primary mode-of-action they exhibit secondary ones, and as a result they interfere with several biochemical systems of the organisms that are exposed to them.

The main goal of my research is to highlight the impact of pesticides on plant metabolism. Based on the advances in technology and bioanalytical tools such as metabolomics, we can study the effects of pesticides on plant’s physiology and products’ nutritional value.

 

EXTERNAL COLLABORATORS

T. Nah (PhD student, collaborator): Fungal metabolomics

N. Sharma (PhD student, collaborator): Insect metabolomics

C. Foti (PhD student, collaborator): Plant metabolomics

D. Gizi (PhD student, collaborator): Plant-pathogen interactions

M. Alasmaey (PhD student, collaborator): Natural product discovery-Metabolomics

M. Xantzigianni (PhD student, collaborator): Plant interactions with abiotic factors

V. Vougieleka (MSc, collaborator): Plant interactions with abiotic factors

E. Naska (MSc, collaborator): Plant-pathogen interactions