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Microbiological tests available to the public

Under the program are available to the public a series of specialized molecular tests for the detection of food borne pathogens in food of animal origin intended for consumption by children. Before shipment of samples please consult with the laboratory. For information and ordering tests click here.

1. Molecular detection of Campylobacter.

Short description: PCR for the detection of part of 16s rRNA gene in bacteria of the genus Campylobacter.

Samples: Any kind of food of animal origin suspected to contain Campylobacter. Campylobacter Campylobacter species are more frequently found in raw poultry products and to a lesser extent in beef, lamp and pork. Infections with Campylobacter have also been reported following consumption of raw milk and shellfish.

Comment: The assay may be incorporated in the diagnostic investigation of foodborn infections. Samples should be shipped as soon as possible in sterile containers.

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2. Molecular detection of enterohaemorragic strains of E. coli (VTEC or EHEC).

Short description: Α combination of three PCR assays for eae (intimin adherence protein), stx1 (shiga toxin 1) and stx2 (shiga toxin 2) genes present in VTEC.

Samples: Any kind of food of animal origin suspected to be contaminated with VTEC. VTEC strains have been more frequently isolated from beef and dairy products. VTEC may be found in fruit and vegetables.

Comment: Τhe assay may be incorporated in the diagnostic investigation of food born infections. Samples should be shipped as soon as possible in sterile containers.

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3. Molecular detection of Listeria.

Short description: PCR assay for the detection of Iap (Invastion associated protein) gene present in bacteria of the genus Listeria.

Samples: Any kind of food of animal origin suspected to be contaminated with Listeria. Bacteria of the genus Listeria may be present in a variety of foods including dairy and meat products as well as ready to eat meals.

Comments: The assay may be incorporated in the diagnostic investigation of foodborn infections. Samples should be shipped as soon as possible in sterile containers.

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4. Molecular detection of Salmonella.

Short description: PCR assay for the detection of InvA (Invasion protein A) gene present in bacteria of the genus Salmonella.

Samples: Any kind of food of animal origin suspected to be contaminated with Salmonella. Bacteria of the genus Salmonella may be present in any kind of preparation due to cross contamination of food but are most commonly associated with pultry, eggs, raw milk and seafood.

Comments: Τhe assay may be incorporated in the diagnostic investigation of foodborn infections. Samples should be shipped as soon as possible in sterile containers.

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5. Molecular detection of Brucella Molecular detection of B. melitensis and B. abortus.

Short description: Combination of two PCR assays for the detection of part of the 16s rRNA gene in Brucella species and the identification of the species B. melitensis and B. aborus.

Samples: Any kind of food of animal origin suspected to be contaminated with Brucella. Brucella infection is usually associated with the consumption of raw milk and dairy products.

Comment: The assay may be incorporated in the diagnostic investigation of foodborn infections. Samples should be shipped as soon as possible in sterile containers.

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6. Molecular detection of Mycobacterium avium subsp. paratuberculosis.

Short description: PCR for the detection of IS900 in Mycobacterium avium subsp. paratuebrculosis.

Samples: Any kind of food of animal origin. Mycobacterium avium subsp. paratuberculosishas been isolated from dairy and meat products (beef, lamb goat, game).

Comment: The role of the bacterium as pathogenic agent has not been yet clarified.

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