
PMG-Research Team
The
Pesticide Metabolomics Group, leaded by
Dr K. A. Aliferis, is hosted by the Laboratory of Pesticide Science
of the Agricultural University of Athens (AUA), Athens, Greece.
The PMG is aiming to provide solutions to major
issues that the agrochemical, agricultural, and food sectors are
currently facing by employing advanced metabolomics and
bioinformatics as its main bioanalytical tools.
Main Research Axes of the PMG’s research:
-
Biostimulant and Biocontrol Research and Development
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Biostimulant formulation
-
Biostimulant, biocontrol and pesticides' mode-of-action discovery applying metabolomics
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Pesticide Research and development
-
Pest and pathogen Resistance to Pesticides
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Ecotoxicology
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Cannabis Research and Development
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Natural product discovery
-
Plant-microbe interactions
-
Food safety and quality applying metabolomics (foodomics)
Current and past Members of the PMG:
Dr K. A. Aliferis (Group Leader)
Editorial Board
Pesticide Biochemistry and Physiology: http://www.journals.elsevier.com/pesticide-biochemistry-and-physiology/
Agrochemicals: https://www.mdpi.com/journal/agrochemicals/editors
LinkedIn:
https://www.linkedin.com/pub/konstantinos-aliferis/24/b41/88Research Gate: http://www.researchgate.net/profile/Konstantinos_Aliferis/info
POSTGRADUATE STUDENTS
A.E. Papadopoulou M.Sc. (Ph.Dc): Fungal endophytes and plant protection
C.N. KEREZOUDIS (Ph.Dc): Biostimulant in Cannabis cultivation
POSTDOCTORAL FELLOWS
I. Kalambokis M.Sc., Ph.D. (Postdoctoral fellow) Metabolomics in pesticde and biostimulant R&D
My
research focuses
on the
development of
combined genetic
engineering-metabolomics
approaches within the context of systems
biology, for the in-depth understudying of the mode(s)-of-action of
fungicides and mechanisms of fungal resistance to fungicides.
Currently, the use of pesticides represents the main crop protection
strategy for the quantitative and qualitative production of
agricultural
products at affordable prices. However, the
effectiveness of pesticides depends on the concentration of the
active ingredient at the subcellular target site. This is the result
of the balance between uptake, probable degradation and excretion of
the active ingredient.One of the most effective control mechanisms of uptake and
excretion, is the active transport through the action of membrane
transport proteins, simply known as transporters. Although excretion
transporters (e.g, ABC and MFS) have to some extent been studied, it
is interesting that this is not the case for transporters that
control the uptake of substances into the cell. Additionally, their
effect on the metabolism and phenotype of organisms is largely
unknown.
The relatively recent development of metabolomics and its
combination with molecular genetic techniques in the context of
systems biology, is able to give answers to the above biological
questions. Results could be exploited in crop protection towards the
development of improved crop protection agents and the assessment of
the risk of fungal resistance to pesticides.
Part of the project is in collaboration with Prof. Diallinas's Aspergillus Genetics Lab, Department of Biology, National and Kapodistrian University of Athens.
Cannabis (Cannabis sativa L.), is a species exhibiting a great potential for applications in various sectors. Nonetheless, the strict regulation of PPPs applications dictate the need for the discovery and/or assessment of alternative PPPs. Within this context, biostimulants represent a source of high potential in Cannabis plant protection. My work focuses on the assessment of the effects of such products on the metabolism of the plant and its cannabinoid content applying metabolomics

Conventional plant protection products (PPPs) represent the backbone of plant protection. Nonetheless, information on their effects on plant metabolism, with the exception of herbicides, is scarce. Additionally, for species such as Cannabis (Cannabis sativa L.) the relevant information is non-existent. Based on the above, aim of my work is the study of the effects of selected fungicides on the metabolism of Cannabis and the biosynthesis of Cannabinoids and terpenoids via metabolomics
E. Pantazi (M.Sc.): Medicinal plants as sources of bioactivity in plant protection
The plant protection sector is facing major issues and challenges.
Therefore, it is very important to discover new, alternative sources
of bioactivity and develop new plant protection agents and
strategies. Within this context, the exploitation of bio-stimulants,
natural products and endophytes in plant protection could provide
valuable solution. In my research, I am evaluating the potential of
extracts of the medicinal plant Aloe barbadensis to be used
as a biostimulant in plant protection applying plant metabolomics.
S. Kostopoulou (M.Sc.): Ecotoxicogenomics-Lemna minor L.
Ecotoxicological and toxicological studies represent key parts of
the research that is required for the development of pesticides.
Currently, results of toxicological studies have indicated endocrine
disrupting activity of some of the most widely applied pesticides.Ecotoxicological and toxicological studies represent key parts of
the research that is required for the development of pesticides.
Currently, results of toxicological studies have indicated endocrine
disrupting activity of some of the most widely applied pesticides.
E. Fotopoulou (M.Sc.): Toxicogenomics-Neural Stem cells and pesticide toxicity
Among the most challenging topics of
pesticide toxicology is the understanding and assessment of
pesticide toxicity through the study of their effects on the
metabolism of biological systems.
D. Karamanou (M.Sc.): Fungal
resistance-Yeast metabolomics
Our knowledge on increased excretion of the active
ingredients could be significantly improved through the
understanding of the interactions between the main biochemical
pathways involved, the genotype and phenotype of resistant strains.
Our knowledge on increased excretion of the active
ingredients could be significantly improved through the
understanding of the interactions between the main biochemical
pathways involved, the genotype and phenotype of resistant strains.
O. Anestidou (B.Sc.): Wine metabolomics: safety and authentication
N. Mparkolias (B.Sc.): Olive tree plant protection against fungal pathogens using advanced plant protection products
E. Pavlakou (B.Sc.): Cannabis (Cannabis sativa L.) plant protection against Rhizoctonia solani: evaluation of novel sources of bioactivity via phenomics and metabolomics
A. Stergios (B.Sc.): Olive oil metabolomics and study of the bioactivity of olive mill waste (OMW)
The olive oil is one of the most important agricultural products of
Greece, with a significant contribution to the GDP of the country
and to the income of those involved in its production and marketing.
However, quantitative and qualitative losses due to pest and
pathogen infections, as well as issues in the marketing and
distribution networks re4presents obstacles towards the further
development of the sector.
The aim of my research is the development of an integrated
GC/EI/MS-1HNMR metabolomics approach for the monitoring of the
quality of olive oil (adulteration, residues of plant protection
products, traceability) and the study of the potential of olive mill
waste (OMW) from a two-phase system in olive tree plant protection.
E. Lamprakopoulou (B.Sc.): Isolation of cannabis phytopathogens and evaluation of the bioactivity of biological agents against them
Cannabis (industrial and
medicinal)
(Cannabis
sativa
L.)
is an
emerging
cultivation world-wide.
The
legislation on medicinal
cannabis in Greece was
introduced in
May
of
2018.
A large number of pests and pathogen
cause severe quantitative and qualitative yield loses in field and
greenhouse cultivations. Additionally, for the production of
superior quality organic
cannabis and cannabis products the use of chemical plant protection
products is not permitted.
The
aim of my research is the
isolation of
cannabis pathogens from field
cultivations and the
evaluation of
the activity of biological agents
against them.
The management of
olive mill waste (OMW) represents a serious challenge, due to their
high content in organics, organic acids and polyphenols, which are
both phytotoxic and degrade the quality of the environment. Due to
their content in bioactive compounds, they represent rich sources of
bioactivity for potential applications in plant protection.
Within this context,
I am studying the phytotoxicity of OMW water extracts using
Lemna
minor
L. as the model plant. Also, I am evaluating their effect on seed
germination, their phytotoxicity to crop and weed species plants and
their
mycotoxicity
to selected phytopathogenic fungi.
E. Papadopoulou (B.Sc.): New sources of bioactivity in pesticide R&D
The
agrochemical and agricultural sectors are facing great challenges
such as, the development of resistant to pesticides plant pathogens,
the recent banning of many pesticides and the increasing public
concern over food quality and safety. Among the key strategies to
tackle such challenges is the discovery of new sources of
bioactivity or development of new or alternative crop protection
agents.
In
my research, fungitoxicity and phytotoxicity bioassays are conducted
in order to assess the potential of selected nanoparticles to be
used i style="mso-bidi-font-style:normal">per se or as
nanocarriers in nanopesticide formulation. Additionally, the
bioactivity of biochar-a product produced through pyrolysis of plant
residues-against different plant pathogenic fungi and bacteria will
be evaluated, which is within the context of a continuous and close
collaboration with a research team of
McGill University, Montreal, Canada.
My research is focused on the isolation,
identification and study of the pesticide resistance level of fungal
strains isolated from infected olive fruits. Results of my research
will provide insights into the issue and will propose measures for
reducing future yield losses.
The incorrect use of agrochemicals has led to issues such as, the
development of resistant to pesticides pest and pathogens, which in turn dictate the need for the discovery of new sources of bioactivity. The latter should have minimum impact on humans and the environment, and at the same time should offer improved crop protection.o:p>
Within this context, aim of my research is the assessment of the
bioactivity of natural extracts against plant pathogenic fungi and
the study of the effect of pesticides on the metabolism of selected
fungi applying metabolomics.
L. Kotsos
Pesticides are applied in order to protect crops from pest and
pathogens. However, there are increasing indications that crop
protection products affect additionally the physiology and
nutritional value of plants. This is mainly due to the fact that in
addition to their primary mode-of-action they exhibit secondary
ones, and as a result they interfere with several biochemical
systems of the organisms that are exposed to them.
The main goal of my research is to highlight the impact of
pesticides on plant metabolism. Based on the advances in technology
and bioanalytical tools such as metabolomics, we can study the
effects of pesticides on plant’s physiology and products’
nutritional value.
N. Sharma (PhD student, collaborator): Insect metabolomics
C. Foti (PhD student, collaborator): Plant metabolomics
V. Vougieleka (MSc, collaborator): Plant interactions with abiotic factors
E. Naska (MSc, collaborator):
Plant-pathogen interactions