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Financing
EEA 2004-2009 and Hellenic Ministry of Economy and Finance
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Implemented by
Agricultural University of Athens
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Head Professor
John Ikonomopoulos
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Contact
Hit Contact
Molecular detection of Listeria monocytogenes.
Polymerase chain reaction for detection of the gene Iap (Invasion associated protein) in Listeria monocytogenes.
Primers: LIS1A: 5΄- atgaatatgaaaaaagcaac-3΄ Primers: LIS1B: 5΄- ttatacgcgaccgaagccaa-3΄ (Bupert et al. 1999) PCR product: 1403 bp
PCR Reaction mix:
Electrophoresis: Electrophoresis in 1% agarose gel at 100V, for 1 h